Matrix metalloproteinase-3 secretion from human pancreatic periacinar myofibroblasts in response to inflammatory

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Osamu Inatomi1, Akira Andoh, Yuhki Yagi

  • 1Department of Internal Medicine, Shiga University of Medical Science, Otsu, Japan.

Pancreas
|January 2, 2007
PubMed
Abstract

Insights

Human pancreatic myofibroblasts secrete matrix metalloproteinase-3 (MMP-3) when stimulated by inflammatory cytokines IL-17, IL-1beta, and TNF-alpha. This suggests a role for these cells in pancreatic tissue remodeling.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Gastroenterology

Background:

  • Matrix metalloproteinases (MMPs) are implicated in pancreatic disorders.
  • The specific regulation of MMP-3 secretion in the pancreas is not well understood.

Purpose of the Study:

  • To investigate the expression and regulation of MMP-3 in human pancreatic periacinar myofibroblasts.

Main Methods:

  • MMP-3 secretion was measured using enzyme-linked immunosorbent assay.
  • MMP-3 mRNA expression was quantified by real-time polymerase chain reaction.
  • The roles of nuclear factor-kappaB (NF-κB) and mitogen-activated protein kinases (MAPKs) were assessed using specific inhibitors and gene transfer.

Main Results:

  • Interleukin-17 (IL-17), IL-1beta, and tumor necrosis factor-alpha (TNF-α) significantly induced MMP-3 secretion and mRNA expression in pancreatic myofibroblasts.
  • Synergistic increases in MMP-3 secretion were observed with combined cytokine stimulation (IL-17 + IL-1beta or IL-17 + TNF-α).
  • Inhibition of NF-κB and MAPKs pathways blocked cytokine-induced MMP-3 secretion, indicating their crucial roles.

Conclusions:

  • Pancreatic periacinar myofibroblasts actively secrete MMP-3 in response to key inflammatory cytokines.
  • These cells may contribute to extracellular matrix turnover in the pancreas through MMP-3 secretion.

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