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Updated: Jul 17, 2026
Quantitative 31P NMR Analysis of Lignins and Tannins
Published on: August 2, 2021
Hydrolyzable tannins with the hexahydroxydiphenoyl unit and the m-depsidic link: HPLC-DAD-MS identification and model
Panagiotis Arapitsas1, Stefano Menichetti, Franco F Vincieri
1Dipartimento di Scienze Farmaceutiche, Polo Scientifico e Tecnologico, Università degli Studi di Firenze, Via U. Schiff 6, 50019 Sesto Fiorentino (FI), Italy.
Abstract:
This study was designed to develop efficient analytical tools for the difficult HPLC-DAD-MS identification of hydrolyzable tannins in natural tissue extracts. Throughout the study of the spectroscopic characteristics of properly synthesized stereodefined standards, it was observed that the UV-vis spectra of compounds with the m-depsidic link showed a characteristic shoulder at 300 nm, consistent with the simple glucogalloyl esters, whereas compounds with the hexahydroxydiphenoyl (HHDP) unit gave a diagnostic fragmentation pattern, caused by a spontaneous lactonization in the mass spectrometer. These observations were confirmed by HPLC-DAD-MS analyses of tannic acid and raspberry extracts, which are rich in hydrolyzable tannins with the m-depsidic link and the HHDP unit, respectively.
