Comparative study of how low-level laser therapy and low-intensity pulsed ultrasound affect bone repair in rats
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Published on: March 10, 2023
Ana Paula Lirani-Galvão1, Vanda Jorgetti, Orivaldo Lopes da Silva
1Bioengineering Department, University of São Paulo, São Paulo, Brazil. analirani@hotmail.com
Objective:
This study aimed to compare the consequences of low-level laser therapy (LLLT) and low-intensity pulsed ultrasound (LIPUS) on bone repair.
Background Data:
Many studies have assessed the effects of LLLT and LIPUS on bone repair, but a comparison of them is rare.
Methods:
Male Wistar rats (n = 48) with tibial bone osteotomy were used. One group had the osteotomized limb treated with LLLT (GaAlAs laser, 780 nm, 30 mW, 112.5 J/cm(2)) and the second group with LIPUS (1.5 MHz, 30 mW/cm(2)), both for 12 sessions (five times per week); a third group was the control. After 20 days, rats were sacrificed and had their tibias submitted to a bending test or histomorphometric analysis.
Results:
In the bending test, maximum load at failure of LLLT group was significantly higher (p < 0.05). Bone histomorphometry revealed a significant increase in osteoblast number and surface, and osteoid volume in the LLLT group, and a significant increase in eroded and osteoclast surfaces in the LIPUS group.
Conclusion:
LIPUS enhanced bone repair by promoting bone resorption in the osteotomy area, while LLLT accelerated this process through bone formation.
