Automated Tecan programming for bioanalytical sample preparation with EZTecan

This paper reports the first successful automated Tecan (Tecan US, Research Triangle Park, NC) programming for bioanalytical sample analysis with an in-house developed software, called EZTecan. Based on the sample run list in the Watson Laboratory Information Management System (InnaPhase Corp., Philadelphia, PA), EZTecan can generate a Tecan program for bioanalytical sample preparation, including sample transfer, sample dilution, extraction, and reconstitution. Many built-in functions for EZTecan ensure the flexibility, quality, and efficiency of the liquid handling. It takes about 90 min to prepare two 96-well plates from sample transfer to reconstitution. Analytical results shown good inter- and intra-assay accuracy and precision, which meet the +/-15% Good Laboratory Performance acceptance criteria. With EZTecan, bioanalysts can prepare samples without writing a single line of Tecan program.