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Imaging Plasma Membrane Deformations With pTIRFM
Published on: April 2, 2014
Glycosylation and transmembrane topography of bovine chromaffin granule p65
H B Tugal1, F van Leeuwen, D K Apps
1Department of Biochemistry, University of Edinburgh Medical School, Scotland, U.K.
Abstract:
The bovine homologue of p65, a calmodulin-binding protein located in the membranes of synaptic vesicles and endocrine secretory granules, has been studied by the use of monoclonal antibodies directed against this antigen and against dopamine beta-mono-oxygenase. The protein (apparent molecular mass 67 kDa; pI = 5.5-6.2) is partially degraded by treatment with neuraminidase or endoglycosidase F. Trypsin treatment of intact adrenal chromaffin granules or of granule membranes releases a soluble 39 kDa fragment of p65 which corresponds to the whole of its cytoplasmic domain. This domain contains both the epitope for the monoclonal antibody cgm67 and the calmodulin-binding site. The 20 amino acids at the N-terminus of this fragment are identical to part of the rat p65 sequence.
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