Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

IL-25 regulates Th17 function in autoimmune inflammation.

Melanie A Kleinschek1, Alexander M Owyang, Barbara Joyce-Shaikh

  • 1Discovery Research, Schering-Plough Biopharma, Palo Alto, CA 94304, USA.

The Journal of Experimental Medicine
|January 4, 2007
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

IFITM1 differentially regulates antibacterial immunity and immunopathology but is dispensable for antiparasitic responses.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

TGF-β mediates epigenetic control of innate antiviral responses and SIV reservoir size.

Nature immunology·2026
Same author

Development of an Ultrasensitive ELISA Assay for Evaluating HIV-1 Envelope Glycoprotein as a Marker for Targeted Activator of Cell Kill.

Viruses·2026
Same author

An IL-12 partial agonist sustains intratumoral lymphocyte activation and detoxifies systemic IL-12 therapy.

Cell reports·2025
Same author

Immunoglobulin M regulates airway hyperresponsiveness independent of T helper 2 allergic inflammation.

eLife·2025
Same author

The effect of treatment with a non-ionic surfactant vesicular formulation of sodium stibogluconate on host immune responses and serum metabolites in a murine model of <i>Leishmania donovani</i>.

Frontiers in immunology·2025

Interleukin-25 (IL-25) regulates autoimmune inflammation. IL-25 deficiency exacerbates experimental autoimmune encephalomyelitis (EAE) by promoting IL-17 responses, while IL-25 treatment suppresses EAE.

Area of Science:

  • Immunology
  • Autoimmunity
  • Cytokine Biology

Background:

  • Interleukin-25 (IL-25) is an IL-17 family cytokine.
  • IL-25 promotes T helper 2 (Th2) responses.
  • The role of IL-25 in autoimmune inflammation is not fully understood.

Purpose of the Study:

  • To investigate the role of IL-25 in the pathogenesis of experimental autoimmune encephalomyelitis (EAE).
  • To elucidate the mechanisms by which IL-25 regulates IL-17-mediated autoimmune inflammation.

Main Methods:

  • Generation and characterization of IL-25-deficient (il25-/-) mice.
  • Induction and assessment of EAE in wild-type and il25-/- mice.
  • Cytokine profiling (IL-17, IFNgamma, TNF, IL-23, IL-13) in response to IL-25 modulation.

Related Experiment Videos

  • In vivo neutralization of IL-17 and IFNgamma.
  • Evaluation of IL-25 treatment effects in EAE models.

    • Main Results:

    • IL-25-deficient mice exhibit increased susceptibility to EAE.
    • Accelerated EAE in il25-/- mice is linked to elevated IL-23 and increased inflammatory T cells (IL-17, IFNgamma, TNF) in the CNS.
    • IL-17 neutralization, but not IFNgamma neutralization, ameliorated EAE in il25-/- mice.
    • IL-25 treatment completely suppressed EAE in both relapsing-remitting and chronic models.
    • IL-25 treatment induced IL-13 production, which inhibited Th17 responses by downregulating IL-23, IL-1beta, and IL-6 in dendritic cells.

    Conclusions:

    • IL-25 and IL-17 play opposing roles in organ-specific autoimmunity.
    • IL-25 acts as a critical regulator, suppressing Th17-mediated autoimmune inflammation.
    • IL-25 represents a potential therapeutic target for autoimmune diseases.