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Related Experiment Video

Updated: Jul 17, 2026

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System
12:06

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System

Published on: May 12, 2011

Gene expression changes in the retina following optic nerve transection.

Natik Piri1, Jacky M K Kwong, Min Song

  • 1Jules Stein Eye Institute, University of California Los Angeles School of Medicine, Los Angeles, CA 90095, USA. piri@jsei.ucla.edu

Molecular Vision
|January 4, 2007
PubMed
Summary

Optic nerve transection (ONT) in rats reveals significant gene expression changes in the retina. This study identified novel genes and potential targets for inherited glaucoma screening.

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Area of Science:

  • Neuroscience
  • Genomics
  • Ophthalmology

Background:

  • Optic nerve injury triggers molecular changes in the retina.
  • Understanding these changes is crucial for retinal ganglion cell (RGC) survival and disease research.

Purpose of the Study:

  • To profile gene expression in the retina after optic nerve transection (ONT).
  • To identify genes involved in RGC response to injury and potential glaucoma markers.

Main Methods:

  • An animal model of optic nerve axotomy was created.
  • Gene expression profiling was performed using microarray analysis.
  • Real-time quantitative and semiquantitative RT-PCR validated gene expression changes.

Main Results:

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Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS
11:02

Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS

Published on: May 12, 2011

Related Experiment Videos

Last Updated: Jul 17, 2026

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System
12:06

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System

Published on: May 12, 2011

Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS
11:02

Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS

Published on: May 12, 2011

  • Microarray analysis revealed differential expression of hundreds of genes post-ONT.
  • Over 493 genes showed increased expression and 113 genes showed decreased expression (≥1.5 fold).
  • Thirteen differentially regulated genes were mapped to known glaucoma loci, suggesting potential for mutation screening.

    • Conclusions:

    • Gene expression profiles after ONT can identify key RGC-expressed genes.
    • This study provides insights into molecular alterations in the retina following RGC loss.
    • Identified genes may be crucial for RGC function and offer targets for glaucoma research.