Related Experiment Video

Updated: Jan 18, 2026

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice
05:59

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

Published on: May 14, 2020

7.0K

Cross-linking the Leu-8 lymph node homing receptor on B cells inhibits immunoglobulin synthesis

Y Murakawa1, W Strober, S P James

  • 1Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, Md.

Immunologic Research
|January 1, 1991
PubMed

About one half of circulating human B cells express the Leu-8 peripheral lymph node homing receptor that has been implicated in adhesion of lymphocytes and neutrophils to vascular endothelium. A novel and unique function of the Leu-8 antigen has been found in the present study: anti-Leu-8 monoclonal antibody directly inhibits B cell antibody production induced by SAC + IL-2, without affecting B cell proliferation or other early steps of B cell activation. This effect of anti-Leu-8 is unique in that other antibodies that inhibit B cell differentiation are not selective and also inhibit B cell proliferation. The inhibitory effect is not reversed by addition of T cells, monocytes, or recombinant IL-1 or IL-6 and is not blocked by anti-TGF-beta. Thus, the natural ligand(s) for Leu-8 may be capable of transducing regulatory signals that have a selective effect on B cell differentiation.

More Related Videos

Isolating Lymphocytes from the Mouse Small Intestinal Immune System11:28

Isolating Lymphocytes from the Mouse Small Intestinal Immune System

Published on: February 28, 2018

56.5K
Isolation of Murine Lymph Node Stromal Cells05:47

Isolation of Murine Lymph Node Stromal Cells

Published on: August 19, 2014

32.1K

Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
17.3K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
16.1K