Related Experiment Video
Updated: Jul 17, 2026

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
Published on: October 14, 2022
ARR1 directly activates cytokinin response genes that encode proteins with diverse regulatory functions
Masatoshi Taniguchi1, Naokazu Sasaki, Tomohiko Tsuge
1Institute for Chemical Research, Kyoto University, Uji, Kyoto, Japan.
Abstract:
Plant cells respond to cytokinins by changing their gene expression patterns. The histidyl-aspartyl (His-Asp) phosphorelay mediates the signal from cytokinin receptors to type-B response regulators including ARR1, which transactivate cytokinin primary response genes. However, the overall architecture of the signal cascade leading to cytokinin-responsive phenomena is still unclear, mainly because it is not known how the His-Asp phosphorelay is connected to downstream phenomena. To reveal events immediately downstream from the phosphorelay-mediated transcriptional activation, we searched for direct-target genes of ARR1 by exploiting ARR1DeltaDDK-GR, a chimeric transcription factor that transactivates ARR1 direct-target genes in transgenic plants by glucocorticoid induction. We identified 23 direct-target genes, most of which were found to be cytokinin primary response genes. The arr1-1 mutation clearly affected the primary response in at least 17 genes, meaning that they respond primarily to cytokinins through the function of ARR1. The 17 genes encode proteins with diverse functions, including type-A response regulators, cytokinin metabolic enzymes and putative disease resistance response proteins. These results provide novel evidence indicating that the His-Asp phosphorelay is connected to diverse regulatory levels of cytokinin-responsive phenomena through ARR1 direct-target genes.
Related Concept Videos
Cell Signaling in Plants
MAPK Signaling Cascades
The JAK-STAT Signaling Pathway
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
TGF - β Signaling Pathway
Regulation of the Unfolded Protein Response

