Related Experiment Video
Updated: Jul 17, 2026
Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery
Published on: April 26, 2024
Efficient adsorption of tumor necrosis factor with an in vitro set-up of the microspheres-based detoxification system
Viktoria Weber1, Jens Hartmann, Ingrid Linsberger
1Center for Biomedical Technology, Danube University Krems, Krems, Austria. viktoria.weber@donau-uni.ac.at
Background:
The Microsperes-Based Detoxification System (MDS) represents a flexible therapeutic option for various diseases, depending on the specificity of the adsorbents applied. A potential application of the MDS is the supportive therapy of sepsis.
Methods:
Microadsorbents for tumor necrosis factor (TNF) were prepared by immobilization of anti-TNF antibodies on cellulose (1-10 microm) and applied in an experimental set-up using a pool of human plasma (1 liter) spiked with TNF (800 pg/ml) and its soluble receptors (1,000 pg/ml each). Removal of TNF was compared using a plasma filter and the albumin-permeable filter Albuflow.
Results:
Addition of 4 (2) g of adsorbent to the filtrate circuit reduced TNF concentrations in the pool by 80% (64%). Removal rates did not differ significantly for the different filters. TNF adsorption was not influenced by the presence of excess TNF receptors.
Conclusions:
Concentrations of mediators can be efficiently modulated with the MDS using small quantities of specific adsorbents.
