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Monoclonal antibody to the prostate specific antigen
F Donn1, T Bruns, L von Meyerrinck
1Urologische Abteilung, Marienkrankenhaus Hamburg, Germany.
Andrologia
|January 1, 1990
Summary
Researchers developed a monoclonal antibody, anti-p34, that targets a prostate specific antigen (PSA). This antibody shows high specificity for prostate tissues and inhibits PSA binding, aiding in prostate cancer research.
Area of Science:
- Immunology
- Oncology
- Biochemistry
Background:
- Prostate-specific antigen (PSA) is a key biomarker in prostate cancer diagnostics.
- Development of specific antibodies is crucial for accurate detection and research.
- Benign prostatic hyperplasia (BPH) shares some characteristics with prostate cancer, necessitating specific markers.
Purpose of the Study:
- To generate a monoclonal antibody that specifically identifies a prostate-specific antigen.
- To characterize the molecular weight and tissue distribution of the identified antigen.
- To evaluate the antibody's reactivity with PSA and its potential in immunoassays.
Main Methods:
- Somatic cell hybridization using mouse myeloma and spleen cells from immunized BALB/c mice.
- Screening of hybridomas via immunoperoxidase staining on human prostate and non-prostate tissues.
- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis.
- Radioimmunoassay (RIA) to assess antibody binding inhibition.
Main Results:
- One monoclonal antibody, designated anti-p34, was identified, recognizing a prostate-specific antigen.
- The antigen presented as a single band of 34 kDa on SDS-PAGE and Western blot.
- Immunoreactivity was localized to the epithelial lining and secretions of normal prostate, BPH, and prostate cancer tissues.
- Anti-p34 antibody demonstrated significant inhibition (80-90%) of Anti-PSA antibody binding to PSA in RIA.
Conclusions:
- The anti-p34 monoclonal antibody specifically targets a 34 kDa prostate-specific antigen.
- This antibody exhibits high specificity for prostate tissues across different conditions (normal, BPH, cancer).
- Anti-p34 antibody recognizes an epitope on the PSA molecule and can interfere with PSA-antibody binding, suggesting its utility in diagnostic and research applications.