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Related Concept Videos

RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Using Solution NMR to Characterize Biomolecular Condensates Under Biphasic Conditions
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Nutrient starvation promotes condensin loading to maintain rDNA stability.

Chi Kwan Tsang1, Hong Li, Xf Steven Zheng

  • 1Department of Pharmacology, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.

The EMBO Journal
|January 5, 2007
PubMed
Summary

Nutrient starvation causes condensin to load onto ribosomal DNA (rDNR) in yeast, preventing its instability. This highlights condensin

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Area of Science:

  • Cellular biology
  • Genomics
  • Molecular biology

Background:

  • Nutrient starvation or rapamycin treatment induces ribosomal DNA (rDNA) array condensation and nucleolar contraction in budding yeast.
  • The target of rapamycin (TOR) pathway regulates these cellular processes.

Purpose of the Study:

  • To investigate the role of condensin in rDNA condensation and stability during nutrient starvation.
  • To elucidate the regulatory mechanisms controlling condensin localization to the rDNA locus.

Main Methods:

  • Budding yeast models
  • Rapamycin treatment
  • Histone deacetylase activity assays
  • Chromatin immunoprecipitation
  • Microscopy

Main Results:

  • Condensin is rapidly relocated into the nucleolus and loaded onto rDNA repeats under nutrient-limiting conditions.
  • Rpd3-dependent histone deacetylation is essential for condensin relocalization and loading to the rDNA array.
  • Rapamycin treatment inhibits rDNA transcription and promotes condensin loading.
  • Inhibition of rDNA transcription without condensin loading results in rDNA instability.

Conclusions:

  • Condensin plays a crucial role in maintaining rDNA stability during nutrient starvation.
  • Histone modification regulates condensin targeting to the rDNA locus.
  • Condensin's enrichment prevents rDNA instability, revealing a novel role in regional genomic stability.