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Updated: Jul 17, 2026

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Published on: October 7, 2020
Isolation and characterization of thermotolerant bacterium utilizing ammonium and nitrate ions under aerobic
Shinji Takenaka1, Qi Zhou, Ampin Kuntiya
1Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Rokko, Kobe, Japan.
Abstract:
A thermotolerant bacterium, identified as Bacillus licheniformis, completely utilized 0.1% (w/v) NH(4)NO(3) at 30 and 50 degrees C under aerobic condition. The addition of 0.5 mM Fe(2+) to the NH(4)NO(3 )medium markedly promoted the utilization of NH (4) (+) and NO (3) (-). At 50 degrees C, of total nitrogen originally provided, 24% was taken up into the cells and 20% remained in the culture supernatant. Residual nitrogen (56%) was probably removed into the atmosphere. The cell extracts contained enzymes involved in denitrification. GC-MS demonstrated that NH (4 ) (15) NO(3) had been converted to (15)N(2)O. These results indicate that the strain has denitrification ability under aerobic condition.
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