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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
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Analysis of mouse development with conditional mutagenesis.

M Lewandoski1

  • 1Laboratory of Cancer and Developmental Biology, NCI-Frederick, National Institutes of Health, Frederick, MD 21702-1201, USA. mlewandoski@mail.ncifcrf.gov

Handbook of Experimental Pharmacology
|January 6, 2007
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Summary

Conditional gene expression systems have revolutionized mouse developmental studies. These advanced technologies enable precise control over gene activity, significantly enhancing our understanding of gene regulation in development and disease.

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Area of Science:

  • Molecular Embryology
  • Developmental Biology
  • Gene Regulation

Background:

  • Mouse molecular embryology is crucial for understanding gene regulation in development and disease.
  • Conditional gene expression systems have advanced precision in analyzing these mechanisms over the past 15 years.

Purpose of the Study:

  • To review the application of conditional gene expression technologies in mouse development.
  • To highlight recent innovations and experimental designs for studying mouse embryos.

Main Methods:

  • Conditional control of gene expression via site-specific DNA recombination.
  • Transcriptional transactivation for gene silencing or activation.

Main Results:

  • Conditional systems offer unprecedented precision in analyzing gene function during mouse development.
  • Innovations in experimental design facilitate detailed study of the mouse embryo.

Conclusions:

  • Conditional gene expression technologies are indispensable tools in modern mouse developmental research.
  • These methods provide critical insights into gene regulation underlying development and disease processes.