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Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions
Published on: September 10, 2021
Infective disorders of the gastrointestinal tract
1Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA. lampslauraw@uams.edu
Abstract:
Gastrointestinal infections are a major cause of morbidity and mortality worldwide. Infectious organisms are often recovered by microbiological methods, but surgical pathologists may play a very valuable role in diagnosis. This review will focus on infective disorders of the gastrointestinal tract with an emphasis on enterocolitides caused by food- and water-borne pathogens. Diagnostic histological features of selected enteric infections will be emphasized, including those that mimic other inflammatory conditions of the gut (such as ischaemia or idiopathic inflammatory bowel disease), along with available diagnostic methods that can aid in diagnosis.
Insights
Surgical pathologists aid in diagnosing gastrointestinal infections, particularly enterocolitis from food/water pathogens. Histological features are key, even when mimicking other gut conditions.
Area of Science:
- Gastroenterology
- Pathology
- Infectious Diseases
Background:
- Gastrointestinal infections cause significant global morbidity and mortality.
- Microbiological methods are standard for pathogen recovery.
- Surgical pathology offers valuable diagnostic insights.
Purpose of the Study:
- To review infective gastrointestinal disorders, focusing on food- and water-borne enterocolitis.
- To highlight diagnostic histological features of enteric infections.
- To discuss diagnostic methods aiding pathologists.
Main Methods:
- Review of literature on gastrointestinal infections and pathology.
- Emphasis on histological findings in enterocolitis.
- Discussion of diagnostic techniques for enteric pathogens.
Main Results:
- Surgical pathology provides crucial diagnostic information for gastrointestinal infections.
- Histological features can identify specific food- and water-borne pathogens.
- Certain infections present with features mimicking other gastrointestinal inflammatory diseases.
Conclusions:
- Pathologists play a vital role in diagnosing gastrointestinal infections.
- Histopathology is essential for identifying enteric pathogens and differentiating mimics.
- Integrated diagnostic approaches enhance patient care for infectious enterocolitis.
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