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Updated: Jul 17, 2026
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Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
Global gene expression profiling of preimplantation embryos
Toshio Hamatani1, Minoru Sh Ko, Mitsutoshi Yamada
1Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan. t-hama@sc.itc.keio.ac.jp
Human Cell
|January 6, 2007
Summary
Early mammalian development involves key gene activation events. Maternal RNA translation is crucial for initiating zygotic genome activation (ZGA), triggering subsequent gene expression waves for embryonic development.
Area of Science:
- Developmental Biology
- Genomics
- Molecular Biology
Background:
- Preimplantation development is critical for mammalian reproduction, involving oocyte-to-embryo transition, compaction, lineage specification, and implantation.
- The small size and limited quantity of preimplantation embryos pose challenges for molecular analysis.
Purpose of the Study:
- To investigate global gene expression patterns during preimplantation development.
- To elucidate the regulatory cascade of gene activation from maternal factors to embryonic genome activation.
Main Methods:
- Global gene expression profiling using RNA amplification and microarray platforms on preimplantation embryos.
- Analysis of gene expression in embryos treated with transcription or translation inhibitors.
Main Results:
- Identified distinct patterns of maternal RNA degradation and embryonic gene activation, including two major transcription waves: zygotic genome activation (ZGA) and mid-preimplantation gene activation (MGA).
- Demonstrated that maternal RNA translation is essential for initiating ZGA, establishing a gene activation cascade: maternal RNA/protein → ZGA genes → MGA genes.
Conclusions:
- Gene expression profiling accelerates the understanding of molecular mechanisms underlying totipotency, pluripotency, and early mammalian development.
- Maternal factors play a crucial role in initiating the cascade of embryonic gene activation during preimplantation development.
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