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[PPARalpha agonist--fenofibrate inhibits LPS-induced tissue factor expression in THP-1 cells]
Chun-Xia Dong1, Yu Hu, Hua-Fang Wang
1Institut of Hematology, Union Hospital, Tongji Medical College, Huazhong Univesity of Science and Technology, Wuhan 430022, China.
Abstract:
This study was aimed to investigate the influence of PPARalpha agonist on the expression of TF (tissue factor) in THP-1 cells. THP-1 cells were pretreated with different concentrations of PPARalpha agonist (fenofibrate) for definite time. Lipopolysaccharide (LPS)-induced TF mRNA and protein levels were detected by RT-PCR and Western blot respectively. The results showed that fenofibrate decreased tissue factor protein and mRNA expression in supernatants of LPS-stimulated human monocytes in a concentration-dependent manner (P < 0.05 - 0.01, n = 5). It is concluded that fenofibrate inhibit TF expression induced by LPS in THP-1 cells, which may be involved in the anti-atherosclerotic effects of PPARalpha agonist.
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