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Fast data preprocessing for chromatographic fingerprints of tomato cell wall polysaccharides using chemometric
Bernard Quéméner1, Dominique Bertrand, Isabelle Marty
1INRA, UR Biopolymères, Interactions, Assemblages, rue de la Géraudière, BP 71627, 44316 Nantes Cedex 03, France.
Journal of Chromatography. A
|January 6, 2007
Summary
Tomato fruit texture is linked to cell wall chemistry. Firm tomato genotypes (LCX, CER) have more glucuronoxylans and pectic arabinan side chains, while mealy types (LC9) have more pectic galactan side chains, indicating key gene locations.
Area of Science:
- Plant biology
- Biochemistry
- Genetics
Background:
- Cell wall polysaccharides significantly influence fruit texture.
- Variability in tomato pericarp cell wall chemistry affects fruit quality traits.
Purpose of the Study:
- To characterize cell wall polysaccharide chemistry in tomato fruit pericarp.
- To correlate chemical profiles with fruit texture traits in different tomato genotypes.
- To identify quantitative trait loci (QTLs) associated with cell wall biosynthesis.
Main Methods:
- Enzymatic degradation of cell wall polysaccharides.
- High-performance anion-exchange chromatography (HPAEC) coupled with mass spectrometry.
- Multivariate analysis including baseline correction and principal component analysis (PCA).
Main Results:
- Firm genotypes (LCX, CER) exhibited higher proportions of glucuronoxylans and pectic arabinan side chains.
- Mealy genotype (LC9) showed the highest content of pectic galactan side chains.
- QTLs on chromosomes 1, 2, 4, and 9 were associated with glucuronoxylan and pectic neutral side chain metabolism.
Conclusions:
- Cell wall polysaccharide composition is a key determinant of tomato fruit texture.
- Specific QTLs play a crucial role in regulating the biosynthesis and metabolism of cell wall components.
- Understanding these genetic and biochemical links can aid in breeding tomatoes with desired textural properties.
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