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A monoclonal antibody sandwich immunoassay for serum amyloid A (SAA) protein
T L McDonald1, A Weber, J W Smith
1Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-6495.
Journal of Immunological Methods
|November 22, 1991
Abstract:
An antibody sandwich immunoassay using two purified rat monoclonal antibodies to human serum amyloid A was developed and used to measure serum amyloid A in human serum. The assay was specific, sensitive, reproducible, and reliable and does not require denaturation of the specimen prior to assay. Serum amyloid A purified by hydrophobic interaction chromatography of acute phase human serum afforded a reliable standard for the assay. A significant (r = 0.69) correlation for SAA and C reactive protein values was found for 180 patient samples analyzed.