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Related Experiment Videos

Nucleolar marker for living cells.

Robert M Martin1, Gisela Tünnemann, Heinrich Leonhardt

  • 1Max Delbrück Center for Molecular Medicine, Robert-Roessle-Str. 10, 13125 Berlin, Germany.

Histochemistry and Cell Biology
|January 6, 2007
PubMed
Summary
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Researchers developed a fluorescent peptide for rapid nucleoli labeling in living cells. This cell-penetrating peptide offers a stable, non-toxic method for visualizing this crucial subnuclear structure.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Increasing demand for precise subcellular structure labeling in live-cell imaging.
  • Need for efficient and specific markers for nucleoli, key sites of ribosome biogenesis.

Purpose of the Study:

  • To develop and validate a novel fluorescently labeled cell-penetrating peptide for rapid nucleoli labeling in living cells.
  • To assess the peptide's efficacy, specificity, stability, and impact on cellular functions.

Main Methods:

  • Synthesis of a fluorescently labeled ten-amino-acid cell-penetrating peptide.
  • Application of the peptide to various living cell types.
  • Validation using actinomycin D treatment, B23 protein and fibrillarin labeling.
  • Co-labeling experiments with fluorescent proteins and DNA dyes.

Related Experiment Videos

  • Assessment of label stability, cellular viability, proliferation, and rDNA transcription over 24 hours.

    • Main Results:

    • The peptide efficiently crossed cell membranes and localized to the nucleoli.
    • Localization confirmed to the granular component of the nucleolus.
    • Labeling compatible with various fluorophores and post-labeling fixation/staining.
    • High label stability over 24 hours with no adverse effects on cell viability, proliferation, or rDNA transcription.

    Conclusions:

    • The developed fluorescent peptide is a valuable, fast, and easy-to-use molecular tool for live-cell nucleoli imaging.
    • It enables specific and stable labeling of nucleoli across different cell types without compromising cellular health.
    • This marker facilitates advanced studies in molecular and cell biology requiring nucleolar visualization.