Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Visualization of RNA transcription and processing.

A L Beyer1, Y N Osheim

  • 1Department of Microbiology, University of Virginia, Charlottesville 22908.

Seminars in Cell Biology
|April 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RNA polymerase II elongation factors Spt4p and Spt5p play roles in transcription elongation by RNA polymerase I and rRNA processing.

Proceedings of the National Academy of Sciences of the United States of America·2006
Same author

EM visualization of transcription by RNA polymerase II: downstream termination requires a poly(A) signal but not transcript cleavage.

Molecular cell·1999
Same author

Separable roles in vivo for the two RNA binding domains of Drosophila A1-hnRNP homolog.

RNA (New York, N.Y.)·1998
Same author

EM visualization of transcriptionally active genes after injection into Xenopus oocyte nuclei.

Methods in cell biology·1997
Same author

Altered levels of the Drosophila HRB87F/hrp36 hnRNP protein have limited effects on alternative splicing in vivo.

Molecular biology of the cell·1996
Same author

Metazoan rDNA enhancer acts by making more genes transcriptionally active.

The Journal of cell biology·1996

This study reveals that RNA splicing often begins during transcription and finishes before polyadenylation in Drosophila genes. However, polyadenylation site cleavage can occur after transcription for some genes.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Understanding gene expression requires visualizing transcription and RNA processing in real-time.
  • The Miller spreading technique offers a unique method for ultrastructural analysis of active genes.

Purpose of the Study:

  • To ultrastructurally analyze transcription, ribonucleoprotein assembly, and early RNA processing events on Drosophila melanogaster Pol II genes.
  • To investigate the temporal relationship between splicing, polyadenylation, and transcription.

Main Methods:

  • Utilized the Miller spreading technique for electron microscopic visualization.
  • Analyzed transcriptionally-active chromatin from Drosophila melanogaster.

Main Results:

Related Experiment Videos

  • Observed that splicing initiates co-transcriptionally as a general rule.
  • Found that splicing is frequently completed before polyadenylation.
  • Demonstrated that cleavage at poly(A) sites occurs post-transcriptionally for specific genes.

Conclusions:

  • The findings challenge previous assumptions about the sequential order of RNA processing events.
  • Co-transcriptional splicing is a predominant mechanism in Drosophila.
  • Post-transcriptional cleavage at poly(A) sites indicates a more complex regulatory process than previously understood.