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Updated: Jul 17, 2026

mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
BcMF11, a putative pollen-specific non-coding RNA from Brassica campestris ssp. chinensis
Jiang-Hua Song1, Jia-Shu Cao, Xiao-Lin Yu
1Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou 310029, PR China.
Abstract:
A full-length cDNA, BcMF11, has been cloned from Chinese cabbage (Brassica campestris L. ssp. chinensis Makino) using rapid amplification of the cDNA ends (RACE) based on a pollen-specific cDNA fragment (DN237921). The BcMF11 cDNA has a total length of 828bp with poly (A) tail. Analysis of the sequence demonstrated that BcMF11 is a novel non-coding RNA which has no prominent open reading frame (ORF) or coding capacity. No significant similarities were observed between BcMF11 and previously published sequences in GenBank. Transcription analysis indicated that BcMF11 is a novel pollen-specific ncRNA and may be involved in the pollen development of Chinese cabbage.

