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Updated: Aug 14, 2026

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus
Published on: January 12, 2015
Digoxigenin as a probe label for in situ hybridization on skeletal tissues
Y S Bland1, M A Critchlow, D E Ashhurst
1Department of Anatomy, St George's Hospital Medical School, Tooting, London, UK.
Abstract:
Non-specific staining was encountered using digoxigenin-labelled cDNA probes for in situ hybridization on sections of skeletal tissues. This staining was most pronounced in cartilaginous matrices. Experimental procedures indicate that the background staining is caused by antibody-binding to hydrophobic sites in the tissues revealed by proteolytic permeabilization. A protocol for minimizing this background is described.
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