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Related Experiment Video

Updated: Jul 17, 2026

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Published on: January 3, 2014

[Oxidase gene from sweetpotato].

Zhihua Liao, Rong Chen, Min Chen

Molekuliarnaia Biologiia

|January 11, 2007

Summary

Researchers cloned and characterized a new polyphenol oxidase (PPO) gene in sweetpotato, IbPPO. This enzyme causes browning, and understanding its expression is key for developing non-browning sweetpotato varieties.

Area of Science:

Plant Biochemistry
Molecular Biology
Genetics

Context:

Enzymatic browning in sweetpotato, caused by polyphenol oxidase (PPO), reduces product quality and market value.
Understanding the genetic basis of PPO is crucial for developing strategies to mitigate browning.

Purpose:

To clone and characterize a novel PPO cDNA from sweetpotato, designated IbPPO.
To analyze the structural features and expression patterns of IbPPO.

Summary:

A full-length IbPPO cDNA (1984 bp) was cloned, encoding a 588-amino acid protein. IbPPO is an intron-free gene with homology to plant PPOs, featuring plastidial transit peptides and conserved copper-binding motifs.
Structural modeling revealed a typical PPO structure with a central copper-binding catalytic site.
Semi-quantitative RT-PCR showed IbPPO expression in all sweetpotato tissues, with highest levels in veins and storage roots.

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Published on: March 29, 2019

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Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

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Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

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Published on: March 29, 2019

Impact:

Provides a molecular basis for understanding enzymatic browning in sweetpotato.
Facilitates the development of genetically engineered, non-browning sweetpotato varieties.
Offers insights into the evolution and structure of plant PPO enzymes.