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[Induction of specific CTL by DC from axillary lymph node of patients with breast cancer]
Yun-jiang Liu1, Jian-li Zhang, Bao-en Shan
1Department of Surgery, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China. lyj818326@126.com
Aim:
To investigate the capability of the dendritic cells (DC) from axillary draining lymph node of patients with breast cancer to induce the antigen specific CTL.
Methods:
The mononuclear cells were isolated from axillary draining lymph node, and the adherent cells were cultured with rhGM-CSF and rhIL-4 to induce DC. The non-adherent cells were cultured with IL-2 to induce tumor draining lymph node cells(TDLNC). DCs stimulated by the autoallergic breast cancer freeze-thawing antigen were co-cultured with the obtained TDLNC to induce tumor antigen specific CTL.
Results:
The positive percentage of CD1a, CD83, CD86 on DC from freshly isolated mononuclear cells in axillary draining lymph node was 11.0+/-2.3, 26.6+/-5.2 and 33.0+/-6.1, respectively. After co-cultured with rhGM-CSF, rhIL-4, breast cancer freeze-thawing antigen and TNF-alpha, the percentage of CD1a, CD83, CD86 was increased to 50.2+/-5.7, 60.5+/-16.5 and 56.2+/-16.4, respectively, (P<0.01). The percentage of CD8(+) T cells in TDLNC increased from 32.8+/-3.2 to 62.5+/-2.5 after co-cultured with DC-Ag (P<0.01).
Conclusion:
Typical DCs can be induced from the mononuclear cells in axillary draining lymph node after stimulated with cytokine (rhGM-CSF, rhIL-4 and TNF-alpha), which possess strong antigen presentation function and can stimulate TDLNC to proliferate and differentiate into antigen specific CTL.
