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Updated: Jul 17, 2026

Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation
Published on: March 20, 2018
X-ray radiation-induced damage in DNA monitored by online Raman
John E McGeehan1, Philippe Carpentier, Antoine Royant
1EMBL, 6 rue Jules Horowitz, BP 181, 38042 Grenoble CEDEX 9, France. mcgeehan@embl.fr
Abstract:
High-quality Raman spectra have been collected on single DNA crystals kept at 100 K in a flow of cold nitrogen gas prior, during and after macromolecular crystallography (MX) data collection. The non-symmetric bending vibration for C-Br bonds in 8-bromo-2'-deoxyguanosine has been unambiguously identified at 293 cm(-1) in the non-resonant Raman spectra. The breakage of this bond could be monitored by the continuous recording of Raman spectra during X-ray exposure, and its decay versus dose could be directly correlated with the loss of the bromide definition within the electron-density maps as determined with MX.
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