Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of Super-Resolution Deep-Learning Reconstruction and Hybrid Iterative Reconstruction for Coronary Stent Assessment on CTA: A Prospective Multicenter Study.

AJR. American journal of roentgenology·2026
Same author

Optimising Haematopoietic Stem Cell Transplantation: Enhancing Myeloablation Sensitivity and Alleviating Anaemia Using Roxadustat (FG-4592).

Cell proliferation·2026
Same author

A water and land resources multi-objective optimal allocation model based on ecological, economic and social sustainability using NSGA-III.

Scientific reports·2026
Same author

Effects of <i>Microbacterium algeriense</i> C14 on growth and rhizosphere environment of <i>Zinnia elegans</i> under cadmium and nickel stress.

Frontiers in microbiology·2026
Same author

The role of impaired bone marrow Tregs in hematopoietic stem cell depletion for pediatric aplastic anemia probably involves immune privilege.

Blood research·2026
Same author

Correction: Prevalence, mutation distribution, and economic burden of thalassemia in China: a systematic review and regional analysis.

Archives of public health = Archives belges de sante publique·2026

Related Experiment Video

Updated: Jul 17, 2026

Nuclear Transfer into Mouse Oocytes
14:17

Nuclear Transfer into Mouse Oocytes

Published on: November 30, 2006

Establishment of customized mouse stem cell lines by sequential nuclear transfer.

Chunli Zhao1, Ruqiang Yao, Jie Hao

  • 1State Key Laboratory of Reproductive Biology, Chinese Academy of Sciences, Beijing 100080, China.

Cell Research
|January 11, 2007
PubMed
Summary

Therapeutic cloning using nuclear transfer-embryonic stem cells (NT-ESCs) shows potential for regenerative medicine. However, sequential NT-ESCs may not overcome developmental deficiencies from earlier donor generations, impacting live birth potential.

More Related Videos

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
13:36

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT

Published on: September 30, 2010

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
12:59

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Published on: August 20, 2017

Related Experiment Videos

Last Updated: Jul 17, 2026

Nuclear Transfer into Mouse Oocytes
14:17

Nuclear Transfer into Mouse Oocytes

Published on: November 30, 2006

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
13:36

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT

Published on: September 30, 2010

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
12:59

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Published on: August 20, 2017

Area of Science:

  • Reproductive biology
  • Stem cell research
  • Developmental biology

Background:

  • Therapeutic cloning, involving embryonic stem cells (ESCs) from nuclear transfer (NT) embryos, is crucial for regenerative medicine.
  • The potential of NT-ESCs is linked to the reprogramming capacity of donor cells.

Purpose of the Study:

  • To establish and characterize nuclear transfer-ESC (NT-ESC) lines from NT embryos with varying donor cell types and passages.
  • To investigate the developmental potential and reprogramming capacity of sequential NT-ESC lines.

Main Methods:

  • Establishment of forty NT-ESC lines from NT embryos.
  • In vitro characterization of NT-ESCs for pluripotency markers and embryoid body formation.
  • In vivo differentiation assessment.
  • Nuclear transfer experiments using early and late passage R1 donor cells and sequential NT-R1-ESC lines.

Main Results:

  • NT-ESCs expressed pluripotency markers, formed embryoid bodies in vitro, and differentiated into embryonic tissues in vivo.
  • Early passage R1 donor cells yielded live pups, while late passage R1 ESC donors lost reprogramming potential for live birth.
  • Sequential NT-R1-ESC lines, derived from late passage R1 ESC donors, failed to produce live pups when used as nuclear transfer donors.

Conclusions:

  • NT-ESCs possess pluripotency and differentiation capabilities.
  • Developmental deficiencies in donor cells can be propagated through sequential NT-ESC lines, hindering successful therapeutic cloning for live birth.
  • Sequential NT-ESCs may not fully rescue developmental defects from previous donor generations.