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Updated: Jul 17, 2026

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
Published on: February 28, 2019
A neutralizable epitope is induced on HGF upon its interaction with its receptor cMet
Kisu Kim1, Youngmi Hur, En-Kyung Ryu
1Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-gu, Seoul, Republic of Korea.
Abstract:
A new conformational neutralizable epitope is created on heptocyte growth factor (HGF), when it interacts with its receptor, cMet. By immunizing rabbits with HGF-cMet complex, we successfully generated a monoclonal antibody (SFN68) that inhibits HGF-cMet interaction, and blocks the biological function mediated by HGF. To define the epitope, we screened out an epitope-mimicking peptide, KSLSRHDHIHHH, from a phage display of combinatorial peptide library. In molecular mimicry this peptide bound to cMet and inhibited HGF-cMet interaction. No humoral response was induced to this epitope-mimicking peptide when immunization was done with HGF alone.
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