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Published on: May 4, 2021
Adipogenesis in mouse 3T3L1 cells: the effects of Rtl1 over-expression
1CSIRO Livestock Industries, Queensland Bioscience Precinct, 306 Carmody Road, St Lucia, 4067 QLD, Australia. lisa.leeton@csiro.au
Functional & Integrative Genomics
|January 12, 2007
Summary
This study investigated the functional relationship between Rtl1 and Delta-like 1 homolog (Dlk1) genes during adipogenesis. Results indicate that despite coordinated down-regulation, Rtl1 and Dlk1 are not functionally related.
Area of Science:
- Genetics
- Developmental Biology
- Molecular Biology
Background:
- Rtl1 and Dlk1 are paternally expressed genes within an imprinted gene cluster on mouse chromosome 12.
- Dlk1 encodes a protein that negatively regulates adipogenesis, while Rtl1 produces a transcript but no confirmed protein.
- Genes at imprinted loci often exhibit coordinated expression and potential functional interactions.
Purpose of the Study:
- To test the hypothesis that Rtl1 and Dlk1 are functionally related.
- To investigate the roles of Rtl1 and Dlk1 in the context of adipogenesis.
Main Methods:
- Analysis of Rtl1 and Dlk1 mRNA expression patterns during induced adipogenesis in 3T3L1 cells.
- Over-expression of Rtl1 to assess its impact on adipogenesis and Dlk1 expression.
Main Results:
- Both Rtl1 and Dlk1 mRNA levels showed similar down-regulation during induced adipogenesis.
- Over-expression of Rtl1 did not alter the adipogenesis timeline or Dlk1 expression levels.
Conclusions:
- Rtl1 and Dlk1 mRNA expression patterns are coordinately regulated during adipogenesis.
- Despite coordinate expression, Rtl1 and Dlk1 are concluded to be neither directly nor indirectly functionally related.
