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Related Experiment Video

Updated: Jul 17, 2026

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

TRPC2: molecular biology and functional importance.

E Yildirim¹, L Birnbaumer

¹Division of Intramural Research, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, 111 T.W. Alexander Drive, Research Triangle Park, NC 27709, USA.

Handbook of Experimental Pharmacology

|January 16, 2007

Summary

Canonical transient receptor potential 2 (TRPC2) channels are crucial for Ca2+ entry in sperm and vomeronasal sensory neurons. Despite its loss in humans, TRPC2 mediates key physiological roles, including pheromone detection and acrosome reactions.

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Last Updated: Jul 17, 2026

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Published on: March 5, 2019

Area of Science:

Ion channel research
Neuroscience
Reproductive biology

Background:

Canonical transient receptor potential (TRPC) channels are nonselective cation channels permeable to Ca2+.
TRPC2, a unique TRPC channel, is lost in humans and Old World monkeys but plays vital roles in sperm and the vomeronasal organ (VNO).

Purpose of the Study:

To elucidate the physiological functions of TRPC2 channels.
To understand the role of TRPC2 in sperm function and vomeronasal sensory perception.

Main Methods:

Utilized immunocytochemistry and gene deletion studies.
Investigated TRPC2's role in sperm-oocyte interaction and pheromone signaling in the VNO.

Main Results:

TRPC2 mediates Ca2+ entry essential for the acrosome reaction in sperm.
In the VNO, TRPC2 acts as the transduction channel for pheromone detection via V1R and V2R receptors.
Distinct VNO pathways involving TRPC2 mediate male aggressive behavior and mating partner recognition.

Conclusions:

TRPC2 is a critical ion channel for reproductive functions and sensory processing of pheromones.
Despite its evolutionary loss in primates, TRPC2's functions highlight conserved mechanisms in mammalian physiology.