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Related Experiment Video

Updated: Jul 17, 2026

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

TRPC6.

A Dietrich¹, T Gudermann

¹Institut für Pharmakologie u. Toxikologie, FB. Medizin, Philipps-Universität Marburg, 35033 Marburg, Germany. dietrica@staff.uni-marburg.de

Handbook of Experimental Pharmacology

|January 16, 2007

Summary

Transient Receptor Potential Canonical 6 (TRPC6) channels are calcium-permeable ion channels found in various cells. TRPC6 plays roles in smooth muscle contraction and kidney podocyte function, but other physiological roles remain unclear.

Area of Science:

Molecular Biology
Physiology
Cell Biology

Background:

Transient Receptor Potential Canonical 6 (TRPC6) is a calcium-permeable, non-selective cation channel.
TRPC6 is expressed in the brain, kidney, immune cells, blood cells, and smooth muscle tissues.
TRPC6 channels exhibit a doubly rectifying current-voltage relationship and are more permeable to Ca2+ than Na+.

Purpose of the Study:

To elucidate the physiological roles of TRPC6 channels.
To investigate the biophysical properties and regulation of TRPC6 channels.
To explore the involvement of TRPC6 in various cell types and tissues.

Main Methods:

Heterologous expression of channel homomers.
Electrophysiological recordings to characterize current-voltage relationships.

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Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

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Studies using TRPC6 knockout (TRPC6-/-) mice.

Main Results:

TRPC6 channels are directly activated by diacylglycerol (DAG) and modulated by phosphorylation.
Extracellular Ca2+ inhibits, while intracellular Ca2+/calmodulin potentiates TRPC6 activity.
TRPC6 deficiency in mice impacts vascular and pulmonary smooth muscle contraction and kidney podocyte structure.

Conclusions:

TRPC6 channels are crucial for smooth muscle contraction and kidney podocyte integrity.
Further research is needed to fully understand TRPC6 functions in immune, blood, and brain cells.
TRPC6's diverse expression suggests multifaceted physiological roles requiring continued investigation.