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Related Experiment Video

Updated: Jul 17, 2026

Controllable Ion Channel Expression through Inducible Transient Transfection

Controllable Ion Channel Expression through Inducible Transient Transfection

Published on: February 17, 2017

TRPP2 channel regulation.

¹Institute for Molecular and Cellular Anatomy, University of Regensburg, Universitätsstrasse 31, 93053 Regensburg, Germany. ralph.witzgall@vkl.uni-regensburg.de

Handbook of Experimental Pharmacology

|January 16, 2007

Summary

Polycystin-2 (TRPP2), encoded by the PKD2 gene, is implicated in autosomal-dominant polycystic kidney disease. This study details its ion channel properties and location in primary cilia.

Area of Science:

Nephrology
Molecular Biology
Cell Biology

Background:

Autosomal-dominant polycystic kidney disease (ADPKD) is linked to mutations in the PKD2 gene.
Polycystin-2 (TRPP2) is the protein encoded by PKD2.
The subcellular localization and trafficking of Polycystin-2 are debated, with emerging consensus on its presence in primary cilia.

Purpose of the Study:

To introduce cystic kidney diseases and the PKD2 gene.
To discuss the ion channel properties of Polycystin-2 in detail.
To clarify the role of Polycystin-2 in cellular function.

Main Methods:

Review of existing literature on Polycystin-2 and cystic kidney diseases.
Analysis of structural predictions for Polycystin-2.

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Discussion of ion channel properties based on structural and functional data.

Main Results:

Polycystin-2 is a 110 kDa protein with six membrane-spanning domains.
It possesses a pore-forming region between the 5th and 6th transmembrane domains.
Consensus suggests Polycystin-2 localizes to primary cilia, organelles involved in sensory functions.

Conclusions:

The PKD2 gene and its protein product, Polycystin-2, are central to understanding ADPKD.
Polycystin-2 functions as an ion channel with specific structural features.
Its localization in primary cilia highlights the importance of these organelles in kidney physiology and disease.