Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CD4-gp120 interactions.

J S McDougal1, D R Klatzmann, P J Maddon

  • 1Centers for Disease Control, Atlanta, Georgia.

Current Opinion in Immunology
|August 1, 1991
PubMed
Summary
This summary is machine-generated.

Researchers determined the 3D structure of the CD4 binding domain and analyzed mutations in CD4 and gp120. Further studies investigate how this binding impacts viral infection and cellular function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alternative algorithms for human immunodeficiency virus infection diagnosis using tests that are licensed in the United States.

Journal of clinical microbiology·2008
Same author

Resistance to HIV-1 entry inhibitors.

Current drug targets. Infectious disorders·2004
Same author

CCR5 and CXCR4 expression on memory and naive T cells in HIV-1 infection and response to highly active antiretroviral therapy.

Journal of acquired immune deficiency syndromes (1999)·2001
Same author

Cd4+ T cells programmed to traffic to lymph nodes account for increases in numbers of cd4+ T cells up to 1 year after the initiation of highly active antiretroviral therapy for human immunodeficiency virus type 1 infection.

The Journal of infectious diseases·2001
Same author

Human immunodeficiency virus type 1 entry inhibitors PRO 542 and T-20 are potently synergistic in blocking virus-cell and cell-cell fusion.

The Journal of infectious diseases·2001
Same author

Assessment of antibody assays for identifying and distinguishing recent from long-term HIV type 1 infection.

AIDS research and human retroviruses·2001
Same journal

A blind spot of human T cell immunology: epitope specificity in secondary lymphoid organs.

Current opinion in immunology·2026
Same journal

Germinal center responses at barrier organ sites.

Current opinion in immunology·2026
Same journal

Ocular sarcoidosis: from clinical signs to targeted interventions.

Current opinion in immunology·2026
Same journal

On or within: spatial determinants of antigen handling in the nasal turbinates.

Current opinion in immunology·2026
Same journal

Decoding the complexity of intestinal immunity with spatial transcriptomics.

Current opinion in immunology·2026
Same journal

Reconsidering the immunological aspects of solid-phase assays for antiphospholipid antibodies detection.

Current opinion in immunology·2026
See all related articles

Area of Science:

  • Structural biology
  • Virology
  • Immunology

Background:

  • The CD4 molecule is a critical co-receptor for human immunodeficiency virus (HIV) entry.
  • The viral envelope glycoprotein gp120 binds to CD4 on T-helper cells, initiating infection.
  • Understanding the structural basis of this interaction is key to developing interventions.

Purpose of the Study:

  • To elucidate the three-dimensional structure of the CD4 binding domain.
  • To investigate the functional consequences of mutations within the CD4 and gp120 binding sites.
  • To explore secondary changes resulting from gp120-CD4 binding relevant to viral infection and cellular function.

Main Methods:

  • X-ray crystallography or cryo-electron microscopy for structure determination.

Related Experiment Videos

  • Site-directed mutagenesis to analyze binding site interactions.
  • Biochemical assays to assess binding affinity and functional effects.

    • Main Results:

    • The three-dimensional structure of the CD4 binding domain has been determined.
    • Extensive mutational analyses of binding sites on gp120 and CD4 have been completed.
    • The study provides a structural and mutational basis for understanding gp120-CD4 interactions.

    Conclusions:

    • The determined structure and mutational data provide insights into the molecular mechanisms of HIV entry.
    • Further investigation into the consequences of gp120-CD4 binding is ongoing.
    • This research contributes to the understanding of viral pathogenesis and potential therapeutic targets.