Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Kinase-independent function of cyclin E.

Yan Geng1, Young-Mi Lee, Markus Welcker

  • 1Department of Cancer Biology, Dana-Farber Cancer Institute, USA.

Molecular Cell
|January 16, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptional states define dependencies and therapeutic vulnerabilities in head and neck cancer.

NPJ precision oncology·2026
Same author

Observations of the Gas Phase Composition of the 2024 BioLab Industrial Plume in the Atlanta Metropolitan Area.

Environmental science & technology letters·2026
Same author

Fate of isoprene peroxy radical constrains the urban photochemical regime.

Science advances·2026
Same author

Therapeutic scheduling of WEE1 inhibition preserves T cell function and promotes immune control of HPV<sup>+</sup> tumors.

bioRxiv : the preprint server for biology·2026
Same author

An interdisciplinary fetal neonatal neurology collaborative promotes integrative life-course brain health.

Frontiers in neurology·2026
Same author

Subtype-Specific Dependencies and Drug Vulnerabilities Enable Precision Therapeutics in Head and Neck Cancer.

bioRxiv : the preprint server for biology·2025

E-type cyclins are crucial for cell cycle progression. Our study reveals cyclin E facilitates MCM helicase loading independently of its kinase activity, by interacting with CDT1 and MCM proteins.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • E-type cyclins activate cyclin-dependent kinases (CDKs), primarily CDK2, to drive cell-cycle progression.
  • Previous research indicated cyclin E-null cells exhibit defects in MCM helicase loading during G(0) to S phase transition.

Purpose of the Study:

  • To investigate the role of cyclin E in MCM helicase loading and S phase entry.
  • To determine if cyclin E's kinase activity is essential for its function in DNA replication initiation.

Main Methods:

  • Utilized cyclin E-null cells and a kinase-deficient cyclin E mutant.
  • Assessed MCM helicase loading onto chromatin during G(0) to S phase progression.
  • Investigated physical interactions between cyclin E, CDT1, and MCM proteins using co-immunoprecipitation.

Related Experiment Videos

Main Results:

  • A kinase-deficient cyclin E mutant partially restored MCM loading and S phase entry in cyclin E-null cells.
  • Cyclin E localizes to chromatin during G(0) to S phase.
  • Cyclin E interacts physically with CDT1 and MCM proteins, acting between CDT1 and MCM loading.
  • CDT1 loading is normal in cyclin E-null cells, but MCM loading is impaired.

Conclusions:

  • E-type cyclins possess kinase-independent functions in cell-cycle progression.
  • Cyclin E facilitates MCM loading through direct physical interactions with CDT1 and MCM, rather than solely through CDK activation.