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Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging
Published on: June 7, 2014
Effect of surface treatment on diffusion and domain formation in supported lipid bilayers
Kalani J Seu1, Anjan P Pandey, Farzin Haque
1Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-2018, USA.
Abstract:
Supported lipid bilayers are widely used as model systems due to their robustness. Due to the solid support, the properties of supported lipid bilayers are different from those of freestanding bilayers. In this article, we examine whether different surface treatments affect the properties of supported lipid bilayers. It will be shown that depending on the treatment method, the diffusion of the lipids can be adjusted approximately threefold without altering the composition. Additionally, as the bilayer-support interaction decreases, it becomes easier to form coexisting liquid-ordered and liquid-disordered domains. The physical/chemical alterations that result from the different treatment methods will be discussed.
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