Related Experiment Video
Updated: Jul 17, 2026
A Simple Device to Rapidly Prepare Whole Mounts of the Mouse Intestine
Published on: October 27, 2015
Effect of running training on DMH-induced aberrant crypt foci in rat colon
Noriyuki Fuku1, Masako Ochiai, Shin Terada
1Division of Health Promotion and Exercise, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku City, Tokyo 162-8636, Japan.
Purpose:
We examined the effects of treadmill-running training on the induction of aberrant crypt foci (ACF), which is the first step of colon cancer induction, in the colonic mucosa of rats injected with 1,2-dimethylhydrazine (DMH).
Methods:
Four-week-old F344 rats (N = 38) were randomly assigned to training (19 rats) and control (19 rats) groups. After a week, all rats were given DMH (20 mg.kg(-1) body weight) once a week for 2 wk. Running training was started at age 7 wk (speed: 10 m.min(-1), 0% grade, 120 min.d(-1), 5 d.wk(-1)). After 4 wk of training, the rats were sacrificed and the colon was removed, opened, and counted for ACF with 0.2% methylene blue staining.
Results:
Running training resulted in lower body- (P < 0.01) and adipose fat weight (P < 0.05). The numbers of ACF and total AC were significantly lower in the running training group than in the control group (P < 0.05). The occurrences of one, three, and five aberrant crypts per focus were also significantly lower in the running training group than in the control group (P < 0.05). The ratios of total AC/ACF did not significantly differ between the running training and control groups.
Conclusions:
The results of the present investigation suggest that low-intensity running training inhibits the DMH-induced initiation of colon ACF development.
