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Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
Published on: October 3, 2014
Promotion of angiogenesis in tissue engineering: developing multicellular matrices with multiple capacities.
E J Suuronen1, L Muzakare, C J Doillon
1Division of Cardiac Surgery, University of Ottawa Heart Institute and Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
The International Journal of Artificial Organs
|January 16, 2007
Summary
Tissue engineering matrices support multiple cell types and vascularization. Researchers developed fibrin and collagen matrices for cornea-sclera models and cell delivery, advancing regenerative medicine.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Vascular Biology
Background:
- Tissue engineering aims to create complex, multi-tissue organs.
- Optimizing cell differentiation and phenotype maintenance is crucial for engineered tissues.
- The angiogenic process is key for vascularizing engineered constructs.
Purpose of the Study:
- To develop prototype tissue engineered matrices supporting simultaneous growth of diverse cell types.
- To investigate matrix compositions promoting vascular and tube formation.
- To create an in vitro cornea-sclera model with innervation and vascular structures.
Main Methods:
- Examined fibrin-based and collagen-based matrix compositions.
- Incorporated growth factors to promote vascularization.
- Developed a co-culture system using fibrin and collagen hydrogels.
- Evaluated in vivo delivery of endothelial progenitor cells using collagen matrices.
Main Results:
- Fibrin matrices with growth factors supported vascular growth and inflammatory cell invasion.
- A fibrin-collagen matrix model successfully replicated cornea-sclera structures with innervation and vasculature.
- Collagen matrices effectively delivered endothelial progenitor cells to ischemic tissue in vivo, promoting vascular integration.
Conclusions:
- Developed functional tissue engineered matrices for supporting multiple cell types and vascularization.
- Demonstrated potential for in vitro models of complex tissues like the cornea-sclera.
- Highlighted the utility of these matrices for cell transplantation and in vitro studies.
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