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Related Experiment Videos

Versican modulates gap junction intercellular communication.

Wang Sheng1, Haiheng Dong, Daniel Y Lee

  • 1Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada.

Journal of Cellular Physiology
|January 16, 2007
PubMed
Summary
This summary is machine-generated.

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Versican V1 isoform promotes cell transition and enhances intercellular communication by increasing connexin 43 localization in gap junctions. This suggests versican plays a role in coordinating cellular functions.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Versican is a large chondroitin sulfate proteoglycan belonging to the lectican family.
  • Alternative splicing of versican generates at least four isoforms (V0, V1, V2, V3).
  • Previous studies showed versican V1 isoform induces mesenchymal-epithelial transition (MET) in NIH3T3 cells.

Purpose of the Study:

  • To investigate the role of versican V1 isoform in intercellular communication.
  • To determine the effect of versican V1 on gap junction function and connexin protein localization.

Main Methods:

  • Cloning of full-length cDNA for chick versican isoforms V1 and V2.
  • Induction of mesenchymal-epithelial transition in NIH3T3 cells.
  • Analysis of connexin 43 localization and gap junction function using blocking assays.

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Main Results:

  • Versican V1 isoform not only induced MET but also increased intercellular communication.
  • V1 isoform promoted plasma membrane localization of connexin 43, enhancing gap junction function.
  • Gap junctions facilitated the transfer of small molecules and second messengers between cells.

Conclusions:

  • Versican V1 isoform enhances intercellular communication through gap junctions.
  • The regulation of gap junction function by versican suggests a role in coordinating cellular activities.
  • Versican's ability to modulate cell-cell communication highlights its broader biological significance.