Chryseobacterium caeni sp. nov., isolated from bioreactor sludge

Area of Science:

• Microbiology
• Bacterial Taxonomy
• Environmental Biotechnology

Background:

• Bioreactors are crucial for degrading environmental pollutants like cyanide.
• Identifying novel microorganisms within these systems is key to understanding and optimizing biodegradation processes.
• The genus Chryseobacterium comprises Gram-negative bacteria with diverse metabolic capabilities.

Purpose of the Study:

• To taxonomically characterize a novel bacterial isolate from a nickel-complexed cyanide-degrading bioreactor.
• To determine the phylogenetic placement and distinctiveness of the novel strain within the Chryseobacterium genus.
• To propose a new species name for the identified bacterium.

Main Methods:

• Polyphasic taxonomic study including 16S rRNA gene sequencing for phylogenetic analysis.
• Analysis of major fatty acids and respiratory quinones.
• Determination of genomic DNA G+C content.

Main Results:

• Strain N4(T) showed 93.2-95.8% 16S rRNA gene sequence similarity to known Chryseobacterium species, indicating novelty.
• Major fatty acids were iso-C(15:0) and summed feature 4; predominant respiratory quinone was MK-6.
• Genomic DNA G+C content was 38.2 mol%.

Conclusions:

• Strain N4(T) represents a novel species within the genus Chryseobacterium.
• The proposed name for the novel species is Chryseobacterium caeni sp. nov.
• The type strain is N4(T) (=KCTC 12506(T)=CCBAU 10201(T)=DSM 17710(T)).