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Related Concept Videos

Voltammetric Techniques: Pulse Voltammetry01:17

Voltammetric Techniques: Pulse Voltammetry

Differential-pulse voltammetry (DPV) is a type of voltammetry that involves applying a series of voltage pulses to an electrochemical cell while measuring the resulting current. In DPV, the differential pulse or small potential pulses are superimposed on a linear potential sweep. The magnitude of these pulses is typically small, often in the millivolt range. Each voltage pulse lasts a short duration, usually in the order of a few milliseconds, and is applied at regular intervals along the...
Voltammetric Techniques: Cyclic Voltammetry01:10

Voltammetric Techniques: Cyclic Voltammetry

Cyclic voltammetry (CV) is an electrochemical technique used to investigate the redox properties of a chemical species. It involves measuring the current response of an electrochemical cell as a function of the applied potential. The setup for cyclic voltammetry typically consists of a working electrode, a reference electrode, and a counter electrode—all immersed in an electrolyte solution. The working electrode is where the redox reaction of interest occurs, while the reference electrode...
Voltammetric Techniques: Linear-Scan (E vs Time)01:12

Voltammetric Techniques: Linear-Scan (E vs Time)

Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...
Voltammetry: Stripping Methods01:13

Voltammetry: Stripping Methods

Anodic Stripping Voltammetry (ASV), Cathodic Stripping Voltammetry (CSV), and Adsorptive Stripping Voltammetry (AdSV) are electrochemical techniques used to determine trace amounts of analytes in solution. These methods involve applying a potential to an electrode and measuring the resulting current.
Anodic Stripping Voltammetry (ASV)
ASV is used to determine metals and metalloids at trace levels. It involves two steps: deposition and stripping. First, a negative potential is applied to the...

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Related Experiment Video

Updated: Jul 17, 2026

Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine
06:40

Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine

Published on: April 23, 2020

Subsecond detection of physiological adenosine concentrations using fast-scan cyclic voltammetry.

B E Kumara Swamy1, B Jill Venton

  • 1Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904, USA.

Analytical Chemistry
|January 16, 2007
PubMed
Summary

A new microelectrode sensor enables real-time monitoring of adenosine, a key molecule in blood flow and neurotransmission. This advancement aids research into conditions like stroke and ischemia by measuring adenosine levels with high temporal resolution.

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Modeling Fast-scan Cyclic Voltammetry Data from Electrically Stimulated Dopamine Neurotransmission Data Using QNsim1.0
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Modeling Fast-scan Cyclic Voltammetry Data from Electrically Stimulated Dopamine Neurotransmission Data Using QNsim1.0

Published on: June 5, 2017

Related Experiment Videos

Last Updated: Jul 17, 2026

Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine
06:40

Combined Infusion and Stimulation with Fast-Scan Cyclic Voltammetry (CIS-FSCV) to Assess Ventral Tegmental Area Receptor Regulation of Phasic Dopamine

Published on: April 23, 2020

Modeling Fast-scan Cyclic Voltammetry Data from Electrically Stimulated Dopamine Neurotransmission Data Using QNsim1.0
07:41

Modeling Fast-scan Cyclic Voltammetry Data from Electrically Stimulated Dopamine Neurotransmission Data Using QNsim1.0

Published on: June 5, 2017

Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Adenosine plays a crucial role in regulating blood flow and neurotransmission.
  • Adenosine exhibits protective effects in pathological conditions like ischemia and stroke.
  • A real-time sensor is essential for understanding adenosine's physiological roles and receptor activation.

Purpose of the Study:

  • To characterize the detection of physiological adenosine concentration changes using carbon-fiber microelectrodes.
  • To achieve subsecond temporal resolution for in vivo adenosine measurements.
  • To develop a sensor for real-time biological monitoring of adenosine.

Main Methods:

  • Fast-scan cyclic voltammetry (FSCV) was employed with carbon-fiber microelectrodes.
  • An applied potential range of -0.4 to 1.5 V at 400 V/s was used, with measurements every 100 ms.
  • Adenosine oxidation was characterized, and electrode sensitivity and selectivity were assessed.

Main Results:

  • Two distinct adenosine oxidation peaks were observed with T-650 carbon fibers.
  • Detection limits of 15 nM were achieved, which is below basal brain adenosine concentrations.
  • The sensor demonstrated high sensitivity to adenosine over ATP and insensitivity to inosine.

Conclusions:

  • Carbon-fiber microelectrodes coupled with FSCV provide a valuable tool for real-time adenosine monitoring.
  • The developed sensor offers high sensitivity, selectivity, and temporal resolution for biological applications.
  • This technology can significantly advance the study of adenosine's physiological and pathological roles.