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Published on: June 24, 2019
Measurement of lysophospholipase D/autotaxin activity in human serum samples
Kazuhiro Nakamura1, Ryunosuke Ohkawa, Shigeo Okubo
1Department of Clinical Laboratory, The University of Tokyo Hospital, Tokyo, Japan.
Objectives:
Lysophospholipase D (lysoPLD)/autotaxin regulates the blood levels of lysophosphatidic acid (LPA), and we designed a serum lysoPLD assay for use in clinical laboratory testing.
Design And Methods:
LysoPLD activity was assessed based on the amount of choline released with lysophosphatidylcholine as the substrate.
Results And Conclusions:
It was confirmed that the lysoPLD assay can be applied to clinical laboratory testing. The serum lysoPLD activity in healthy female subjects was significantly higher than that in the male subjects.
