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Updated: Jul 17, 2026
11:40
Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
Published on: April 17, 2020
Coupling of rotavirus genome replication and capsid assembly
John T Patton1, Rodrigo Vasquez-Del Carpio, M Alejandra Tortorici
1Laboratory of Infectious Diseases, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Advances in Virus Research
|January 16, 2007
Summary
Reoviridae viruses replicate RNA within viral cores, requiring capsid protein assembly for double-stranded RNA synthesis. This prevents viral RNA detection and host antiviral responses.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Reoviridae viruses possess segmented double-stranded (ds)RNA genomes and cause significant diseases.
- Viral RNA transcription and replication occur within viral cores, mediated by RNA-dependent RNA polymerase (RdRP).
Purpose of the Study:
- To elucidate the mechanism of dsRNA synthesis and replication in Reoviridae, focusing on Rotavirus.
- To understand the role of viral core proteins in regulating viral RNA replication.
Main Methods:
- In vitro studies using Rotavirus RdRP to analyze interactions with viral (+) strand RNAs.
- Investigating the requirement of core capsid protein for the initiation of dsRNA synthesis.
Main Results:
- Rotavirus RdRP recognizes viral (+) strand RNAs but requires core capsid protein for dsRNA synthesis initiation.
- dsRNA synthesis is coupled with capsid packaging, preventing naked dsRNA accumulation.
Conclusions:
- Viral polymerase activity is regulated by capsid assembly, ensuring efficient replication and evasion of host defenses.
- The mechanism prevents the triggering of dsRNA-dependent interferon signaling pathways by naked viral RNA.
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