Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Transducer Mechanism: Enzyme-Linked Receptors

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers

Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Intracellular Ionic Remodeling During Fetal Development of Hereditary Cardiomyopathy of the Hamster.

Pathophysiology : the official journal of the International Society for Pathophysiology·2026

Same author

Resting Cytosolic and Nuclear Reactive Oxygen Species (ROS) Are Regulated by the Basal Activity of ET-1 Receptors in Human Vascular Smooth Muscle Cells.

International journal of molecular sciences·2026

Same author

Exogenous and endogenous antioxidants (ROS) in physiology and pathology of the cardiovascular system.

Canadian journal of physiology and pharmacology·2025

Same author

Pathophysiology of Prediabetes Hyperinsulinemia and Insulin Resistance in the Cardiovascular System.

Biomedicines·2025

Same author

In Vitro Chronic Hyperinsulinemia Induces Remodelling of Vascular Smooth Muscle Cells from Young Men and Women in a Sex Hormone Independent Manner.

Pathophysiology : the official journal of the International Society for Pathophysiology·2025

Same author

Short Communication: Taurine Long-Term Treatment Prevents the Development of Cardiac Hypertrophy, and Premature Death in Hereditary Cardiomyopathy of the Hamster Is Sex-Independent.

Nutrients·2024

Search research articles

Related Experiment Video

Updated: Jul 17, 2026

Hybrid Cell Analysis System to Assess Structural and Contractile Changes of Human iPSC-Derived Cardiomyocytes for Preclinical Cardiac Risk Evaluation

Hybrid Cell Analysis System to Assess Structural and Contractile Changes of Human iPSC-Derived Cardiomyocytes for Preclinical Cardiac Risk Evaluation

Published on: October 20, 2022

NPY regulates human endocardial endothelial cell function.

Dima Abdel-Samad¹, Danielle Jacques, Claudine Perreault

¹Department of Anatomy & Cell Biology, Faculty of Medicine, Université de Sherbrooke, Sherbrooke, 3001-12th Avenue North, Québec, Canada J1H 5N4.

|January 16, 2007

Summary

Endocardial endothelial cells release Neuropeptide Y (NPY), which regulates cardiac function. Activation of NPY receptors on these cells influences calcium levels and cardiomyocyte beating, impacting heart excitation and contraction.

More Related Videos

Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart

Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart

Published on: May 19, 2022

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

Published on: February 3, 2018

Related Experiment Videos

Last Updated: Jul 17, 2026

Hybrid Cell Analysis System to Assess Structural and Contractile Changes of Human iPSC-Derived Cardiomyocytes for Preclinical Cardiac Risk Evaluation

Hybrid Cell Analysis System to Assess Structural and Contractile Changes of Human iPSC-Derived Cardiomyocytes for Preclinical Cardiac Risk Evaluation

Published on: October 20, 2022

Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart

Time-Resolved In Vivo Measurement of Neuropeptide Dynamics by Capacitive Immunoprobe in Porcine Heart

Published on: May 19, 2022

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters

Published on: February 3, 2018

Area of Science:

Cardiovascular Biology
Cellular Physiology
Endothelial Cell Function

Background:

Endocardial endothelial cells (EECs) are increasingly recognized for their role in cardiac regulation.
EECs release cardioactive factors like endothelin-1 (ET-1), Angiotensin II (Ang II), and nitric oxide (NO).
The presence and function of Neuropeptide Y (NPY) receptors in EECs were previously unexplored.

Purpose of the Study:

To investigate the presence and function of NPY receptors in EECs.
To determine if NPY release from EECs plays a role in cardiac function.
To elucidate the mechanisms by which NPY influences EECs and cardiomyocytes.

Main Methods:

Characterization of NPY receptor subtypes (Y(1) and Y(2)) in EECs.
Measurement of intracellular free Ca(2+) levels in EECs upon receptor activation.
Assessment of cardiomyocyte beating frequency in response to EEC stimulation.
Investigation of NPY release from EECs under specific conditions.

Main Results:

EECs express functional Y(1) and Y(2) NPY receptors.
Activation of these NPY receptors increases intracellular Ca(2+) in EECs.
This activation also elevates the beating frequency of cardiomyocytes.
NPY is present in EECs and is released upon increased intracellular Ca(2+).

Conclusions:

NPY, like ET-1, is released from EECs and acts as a cardioactive factor.
NPY signaling in EECs influences both EEC excitation-secretion and cardiomyocyte excitation-contraction coupling.
These findings highlight a novel role for NPY in regulating cardiac function via EECs.