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Rice protein 16KD--a major allergen in rice grain extract
Abstract:
The allergenic activity of Rice protein 16 KD (RP16KD) isolated from water soluble rice proteins was examined by radioallergosorbent test (RAST), RAST inhibition and histamine release assay. All of the 31 sera which showed positive RAST values for rice grain extract were positive for RP16KD RAST. Furthermore, there was a significant correlation (r = 0.56, p less than 0.01) between these RAST values. PR16KD effectively inhibited IgE binding to the rice grain extract disc in RAST inhibition assays using 4 sera with positive RAST values for both antigens. In 17 subjects with positive RAST values for rice grain extract, a significant positive correlation (r = 0.53, p less than 0.05) was found between the maximum percent histamine releases from their leukocytes by rice grain extract and RP16KD. These data strongly suggest that RP16KD is one of the major allergens of rice grain.
Insights
Rice protein 16 KD (RP16KD) is a major allergen in rice grains. Studies confirmed its allergenic activity through RAST, RAST inhibition, and histamine release assays, indicating its significance in rice allergies.
Area of Science:
- Food allergy research
- Immunology
- Protein biochemistry
Background:
- Rice is a staple food globally, and rice allergy is a recognized food hypersensitivity.
- Identifying specific allergenic components is crucial for understanding and managing rice allergy.
Purpose of the Study:
- To investigate the allergenic potential of Rice protein 16 KD (RP16KD), a component of water-soluble rice proteins.
- To determine if RP16KD is a major allergen in rice grains.
Main Methods:
- Radioallergosorbent test (RAST) was used to assess IgE reactivity to RP16KD.
- RAST inhibition assays were performed to evaluate IgE binding inhibition by RP16KD.
- Histamine release assays from leukocytes were conducted to measure cellular responses to RP16KD.
Main Results:
- All tested sera positive for rice grain extract also showed positive RAST values for RP16KD.
- RP16KD significantly inhibited IgE binding to rice grain extract.
- A positive correlation was observed between histamine release induced by rice grain extract and RP16KD.
Conclusions:
- RP16KD demonstrates significant allergenic activity.
- RP16KD is identified as a major allergen in rice grains, contributing to rice allergy.