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Recombinant DNA

Recombinant DNA

Recombinant DNA

Recombinant DNA

Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...

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Related Experiment Video

Updated: Jul 17, 2026

Laboratory Scale Production and Purification of a Therapeutic Antibody

Laboratory Scale Production and Purification of a Therapeutic Antibody

Published on: January 24, 2017

Recombinant therapeutic antibodies.

Stefan Dübel¹

¹Technical University of Braunschweig, Institute of Biochemistry and Biotechnology, Spielmannstr 7, Braunschweig, Germany. s.duebel@tu-bs.de

Applied Microbiology and Biotechnology

|January 17, 2007

Summary

Recombinant antibody technology has transformed antibody therapeutics. This review covers key technologies and future directions in this fast-evolving field.

Area of Science:

Biotechnology
Immunology
Pharmaceutical Science

Background:

Antibody therapeutics have become crucial in modern medicine.
Recombinant DNA technology is fundamental to their production.

Purpose of the Study:

To provide an overview of enabling technologies for recombinant antibody development.
To discuss future prospects in the field of antibody therapeutics.

Main Methods:

Literature review of recent advancements in recombinant antibody technology.
Analysis of current trends and future projections in antibody engineering.

Main Results:

Recombinant antibody technology offers diverse platforms for therapeutic development.

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Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Published on: June 5, 2015

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Last Updated: Jul 17, 2026

Laboratory Scale Production and Purification of a Therapeutic Antibody

Laboratory Scale Production and Purification of a Therapeutic Antibody

Published on: January 24, 2017

Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody

Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody

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Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

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Significant progress has been made in antibody engineering and production.

Conclusions:

The field of antibody therapeutics is rapidly advancing due to recombinant technologies.
Future prospects include novel antibody formats and enhanced therapeutic efficacy.