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M L Colombo1, F Tomé

  • 1Istituto di Scienze Farmacologiche, Università di Milano, Via Balzaretti 9, I-20133 Milano, Italy.

Planta Medica
|October 1, 1991
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Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media