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Alkaloid Production in Relation to Differentiation in Cell and Tissue Cultures of Tabernaemontana pandacaqui

M I Sierra1, R van der Heijden, J Schripsema

  • 1Center for Bio-Pharmaceutical Sciences, Divison of Pharmacognosy, Leiden University. Gorlaeus Laboratoria, P.O. Box 9502, 2300 RA Leiden, The Netherlands.

Planta Medica
|December 1, 1991
PubMed

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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media