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Distribution, clearance, and mortality of environmental pseudomonads in mice upon intranasal exposure

S E George1, M J Kohan, D A Whitehouse

  • 1Health Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina 27711.

Applied and Environmental Microbiology
|August 1, 1991
PubMed

Insights

Intranasal administration of Pseudomonas aeruginosa AC869 at high doses (10(7) CFU) caused persistence and morbidity in mice. Lower doses and Pseudomonas cepacia AC1100 were cleared effectively.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Pharmacokinetics

Background:

  • Pseudomonas aeruginosa and Pseudomonas cepacia are opportunistic pathogens.
  • Understanding bacterial clearance and pathogenicity is crucial for infection control.

Purpose of the Study:

  • To evaluate the intranasal clearance, persistence, and pathogenicity of Pseudomonas aeruginosa AC869 and Pseudomonas cepacia AC1100 in a mouse model.
  • To determine the 50% lethal dose (LD50) of Pseudomonas aeruginosa AC869.

Main Methods:

  • Mice were inoculated intranasally with specific doses of P. aeruginosa AC869 or P. cepacia AC1100.
  • Bacterial clearance was monitored over time.
  • Morbidity and mortality were assessed.
  • The LD50 of P. aeruginosa AC869 was determined.

Main Results:

  • P. cepacia AC1100 (10(8) CFU) and low-dose P. aeruginosa AC869 (10(3) CFU) were rapidly cleared.
  • High-dose P. aeruginosa AC869 (10(7) CFU) persisted for 14 days and exhibited a 50% lethal dose of 2.7 x 10(7) CFU.
  • Slight morbidity was observed with high-dose P. aeruginosa AC869 or P. cepacia AC1100.

Conclusions:

  • High-dose intranasal inoculation of P. aeruginosa AC869 leads to bacterial persistence and potential pathogenicity in mice.
  • P. cepacia AC1100 is cleared more readily than P. aeruginosa AC869 at high doses.
  • These findings highlight dose-dependent effects on bacterial clearance and virulence.

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