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Related Experiment Videos

Peritoneal fluid kinetics during CAPD measured with intraperitoneal dextran 70.

R T Krediet1, D G Struijk, G C Koomen

  • 1Renal Unit, Academic Medical Center Amsterdam, The Netherlands.

ASAIO Transactions
|October 1, 1991
PubMed
Summary

Dextran 70 effectively measures peritoneal fluid kinetics, including transcapillary ultrafiltration and lymphatic absorption, during continuous ambulatory peritoneal dialysis (CAPD). This marker is reliable even with low glucose dialysate concentrations.

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Area of Science:

  • Nephrology
  • Physiology
  • Biomedical Engineering

Background:

  • Peritoneal dialysis (PD) involves fluid and solute exchange across the peritoneal membrane.
  • Accurate measurement of peritoneal fluid kinetics is crucial for optimizing PD therapy.
  • Dextran 70 has been investigated as a potential marker for these measurements.

Purpose of the Study:

  • To simultaneously measure transcapillary ultrafiltration (TCUF), lymphatic absorption rate (LAR), and intraperitoneal volume (IPV) using dextran 70.
  • To assess the utility of dextran 70 as a marker for peritoneal fluid kinetics during CAPD.
  • To correlate fluid kinetics with solute transport parameters.

Main Methods:

  • Intraperitoneal administration of polydisperse dextran 70 in nine CAPD patients.

Related Experiment Videos

  • Measurement of TCUF, LAR, and IPV over a 4-hour dialysis dwell with low glucose (1.36%).
  • Application of Lineweaver-Burke plot for TCUFmax and half-time (t50) calculation; correlation analysis with solute transport (MTC).

    • Main Results:

    • Dextran 70 recovery was high (88 +/- 1%).
    • LAR was 1.30 +/- 0.12 ml/min.
    • TCUF followed a hyperbolic curve, yielding TCUFmax (median 641 mL) and t50 (median 211 min).
    • Measured delta IPV4h correlated strongly with calculated values (r=0.89).
    • IPV changes were influenced by LAR and effective peritoneal surface area (MTC).
    • High solute MTC values correlated with shorter t50.
    • LAR correlated with inulin MTC.
    • Dextran 70 did not affect solute transport.

    Conclusions:

    • Dextran 70 is a valuable marker for measuring peritoneal fluid kinetics (TCUF, LAR, IPV) during CAPD.
    • Its efficacy is maintained even with low dialysate glucose concentrations.
    • The study provides insights into the relationship between fluid dynamics and solute transport in the peritoneal membrane.