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Updated: Jul 17, 2026
Repetitive Transcranial Magnetic Stimulation to the Unilateral Hemisphere of Rat Brain
Published on: October 22, 2016
High-frequency monopolar electrical stimulation of the rat cerebral cortex
Masahiro Oinuma1, Kyouichi Suzuki, Takashi Honda
1Department of Neurosurgery, Fukushima Medical University, Fukushima, Japan. oinuma-nsu@umin.ac.jp
Objective:
Intraoperative monitoring of the motor-evoked potential has been widely used in patients undergoing neurosurgery. Direct stimulation of the brain with high-frequency monopolar stimulation (HFMS) is one of the most common methods to produce motor-evoked potential. We studied the influence of HFMS on the rat cerebral cortex.
Methods:
We applied 1.5, 15, 30, 40, or 50 mA of HFMS to the rat sensorimotor cortex by a short sequence of five monopolar, monophasic, anodal rectangular 500-Hz pulses. We delivered one short five-pulse train 100 times every 5 seconds and examined pre- and post-stimulation electroencephalograms and histological changes at the stimulation site.
Results:
We observed no spike waves after HFMS in any of the rats. There was no change in the power spectrum or frequency content in any of the rats exposed to HFMS. Histologically, there was significant swelling of the dendrites in rats sacrificed immediately after exposure to 40- and 50-mA stimulation; the 50-mA stimulation group also exhibited slight swelling of the mitochondria. These findings were not obtained in any of the rats sacrificed 30 days after stimulation.
Conclusion:
In rats exposed to a stimulation intensity of 30-mA or less, no morphological or electrophysiological changes were observed. However, the possibility that HFMS may affect neural tissue cannot be ruled out.
